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Key Components of Different Plant Defense Pathways Are Dispensable for Powdery Mildew Resistance of the Arabidopsis mlo2 mlo6 mlo12 Triple Mutant.

dc.contributor.authorKuhn, Hannah
dc.contributor.authorLorek, Justine
dc.contributor.authorKwaaitaal, Mark
dc.contributor.authorConsonni, Chiara
dc.contributor.authorBecker, Katia
dc.contributor.authorMicali, Cristina
dc.contributor.authorVer Loren van Themaat, Emiel
dc.contributor.authorBednarek, Paweł
dc.contributor.authorRaaymakers, Tom M.
dc.contributor.authorAppiano, Michela
dc.contributor.authorBai, Yuling
dc.contributor.authorMeldau, Dorothea
dc.contributor.authorBaum, Stephani
dc.contributor.authorConrath, Uwe
dc.contributor.authorFeussner, Ivo
dc.contributor.authorPanstruga, Ralph
dc.date.accessioned2017-12-14T13:15:55Z
dc.date.available2017-12-14T13:15:55Z
dc.date.issued2017
dc.relation.ISSN1664-462X
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/14980
dc.description.abstractLoss of function mutations of particular plant MILDEW RESISTANCE LOCUS O (MLO) genes confer durable and broad-spectrum penetration resistance against powdery mildew fungi. Here, we combined genetic, transcriptomic and metabolomic analyses to explore the defense mechanisms in the fully resistant Arabidopsis thaliana mlo2 mlo6 mlo12 triple mutant. We found that this genotype unexpectedly overcomes the requirement for indolic antimicrobials and defense-related secretion, which are critical for incomplete resistance of mlo2 single mutants. Comparative microarray-based transcriptome analysis of mlo2 mlo6 mlo12 mutants and wild type plants upon Golovinomyces orontii inoculation revealed an increased and accelerated accumulation of many defense-related transcripts. Despite the biotrophic nature of the interaction, this included the non-canonical activation of a jasmonic acid/ethylene-dependent transcriptional program. In contrast to a non-adapted powdery mildew pathogen, the adapted powdery mildew fungus is able to defeat the accumulation of defense-relevant indolic metabolites in a MLO protein-dependent manner. We suggest that a broad and fast activation of immune responses in mlo2 mlo6 mlo12 plants can compensate for the lack of single or few defense pathways. In addition, our results point to a role of Arabidopsis MLO2, MLO6, and MLO12 in enabling defense suppression during invasion by adapted powdery mildew fungi.
dc.languageeng
dc.language.isoeng
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectMLO; camalexin; indole glucosinolates; jasmonic acid; microarray analysis; plant defense; powdery mildew; tryptophan
dc.subject.ddc572
dc.titleKey Components of Different Plant Defense Pathways Are Dispensable for Powdery Mildew Resistance of the Arabidopsis mlo2 mlo6 mlo12 Triple Mutant.
dc.typejournalArticle
dc.identifier.doi10.3389/fpls.2017.01006
dc.type.versionpublishedVersion
dc.bibliographicCitation.volume8
dc.type.subtypejournalArticle
dc.identifier.pmid28674541
dc.bibliographicCitation.articlenumber1006
dc.description.statuspeerReviewed
dc.bibliographicCitation.journalFrontiers in plant science


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