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Development of a DNA Microarray-Based Assay for the Detection of Sugar Beet Root Rot Pathogens

dc.contributor.authorLiebe, Sebastian
dc.contributor.authorChrist, Daniela S.
dc.contributor.authorEhricht, Ralf
dc.contributor.authorVarrelmann, Mark
dc.date.accessioned2019-11-01T12:29:33Z
dc.date.available2019-11-01T12:29:33Z
dc.date.issued2016de
dc.relation.ISSN1943-7684de
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/16585
dc.description.abstractSugar beet root rot diseases that occur during the cropping season or in storage are accompanied by high yield losses and a severe reduction of processing quality. The vast diversity of microorganism species involved in rot development requires molecular tools allowing simultaneous identification of many different targets. Therefore, a new microarray technology (ArrayTube) was applied in this study to improve diagnosis of sugar beet root rot diseases. Based on three marker genes (internal transcribed spacer, translation elongation factor 1 alpha, and 16S ribosomal DNA), 42 well-performing probes enabled the identification of prevalent field pathogens (e.g., Aphanomyces cochlioides), storage pathogens (e.g., Botrytis cinerea), and ubiquitous spoilage fungi (e.g., Penicillium expansum). All probes were proven for specificity with pure cultures from 73 microorganism species as well as for in planta detection of their target species using inoculated sugar beet tissue. Microarray-based identification of root rot pathogens in diseased field beets was successfully confirmed by classical detection methods. The high discriminatory potential was proven by Fusarium species differentiation based on a single nucleotide polymorphism. The results demonstrate that the ArrayTube constitute an innovative tool allowing a rapid and reliable detection of plant pathogens particularly when multiple microorganism species are present.de
dc.language.isoengde
dc.rightsopenAccess
dc.subjectDNA; Microarray-Based Assayde
dc.subject.ddc230
dc.subject.ddc650
dc.titleDevelopment of a DNA Microarray-Based Assay for the Detection of Sugar Beet Root Rot Pathogensde
dc.typejournalArticlede
dc.identifier.doi10.1094/PHYTO-07-15-0171-R
dc.type.versionpublishedVersionde
dc.relation.pISSN0031-949X
dc.bibliographicCitation.volume106de
dc.bibliographicCitation.issue1de
dc.bibliographicCitation.firstPage76de
dc.bibliographicCitation.lastPage86de
dc.type.subtypejournalArticle
dc.identifier.pmid26524545
dc.description.statuspeerReviewedde
dc.bibliographicCitation.journalPhytopathologyde


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