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A Metataxonomic Tool to Investigate the Diversity of Treponema

dc.contributor.authorHallmaier-Wacker, Luisa K.
dc.contributor.authorLüert, Simone
dc.contributor.authorGronow, Sabine
dc.contributor.authorSpröer, Cathrin
dc.contributor.authorOvermann, Jörg
dc.contributor.authorBuller, Nicky
dc.contributor.authorVaughan-Higgins, Rebecca J.
dc.contributor.authorKnauf, Sascha
dc.date.accessioned2019-12-02T13:51:24Z
dc.date.available2019-12-02T13:51:24Z
dc.date.issued2019de
dc.relation.ISSN1664-302Xde
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/16796
dc.description.abstractThe genus Treponema contains a number of human and animal pathogenic as well as symbiotic bacteria that are found in vastly different anatomical and environmental habitats. Our understanding of the species range, evolution, and biology of these important bacteria is still limited. To explore the diversity of treponemes, we established, validated, and tested a novel metataxonomic approach. As the informative nature of the hypervariable regions of the 16S rRNA gene differ, we first analyzed each variable region independently. Considering the in silico results obtained, we established and validated the sequencing of the V4-region of the 16S rRNA gene using known mixtures of Treponema species as well as a selected number of clinical samples. The metataxonomic approach was able to identify Treponema to a near-species level. We demonstrate that using a spirochete-specific enrichment, our method is applicable to complex microbial communities and large variety of biological samples. The metataxonomic approach described provides a useful method to unravel the full diversity and range of Treponema in various ecosystems.de
dc.language.isoengde
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject16S rRNA; Potorous; Treponema; marsupial; metagenomics; metataxonomics; one health; spirochetede
dc.subject.ddc599
dc.titleA Metataxonomic Tool to Investigate the Diversity of Treponemade
dc.typejournalArticlede
dc.identifier.doi10.3389/fmicb.2019.02094
dc.identifier.doi10.3389/fmicb.2019.02094.s001
dc.type.versionpublishedVersionde
dc.relation.eISSN1664-302X
dc.bibliographicCitation.volume10de
dc.type.subtypejournalArticle
dc.identifier.pmid31552004
dc.bibliographicCitation.articlenumber2094de
dc.description.statuspeerReviewedde
dc.bibliographicCitation.journalFrontiers in Microbiologyde


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