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Urine E-cadherin: A Marker for Early Detection of Kidney Injury in Diabetic Patients

dc.contributor.authorKoziolek, Michael
dc.contributor.authorMueller, Gerhard A.
dc.contributor.authorDihazi, Gry H.
dc.contributor.authorJung, Klaus
dc.contributor.authorAltubar, Constanze
dc.contributor.authorWallbach, Manuel
dc.contributor.authorMarkovic, Ivana
dc.contributor.authorRaddatz, Dirk
dc.contributor.authorJahn, Olaf
dc.contributor.authorKaraköse, Hülya
dc.contributor.authorLenz, Christof
dc.contributor.authorUrlaub, Henning
dc.contributor.authorDihazi, Abdelhi
dc.contributor.authorEl Meziane, Abdellatif El
dc.contributor.authorDihazi, Hassan
dc.date.accessioned2020-06-26T13:33:33Z
dc.date.available2020-06-26T13:33:33Z
dc.date.issued2020de
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/17408
dc.description.abstractDiabetic nephropathy (DN) is themain reason for end-stage renal disease. Microalbuminuria as the non-invasive available diagnosismarker lacks specificity and gives high false positive rates. To identify and validate biomarkers for DN, we used in the present study urine samples from four patient groups: diabetes without nephropathy, diabetes withmicroalbuminuria, diabetes withmacroalbuminuria and proteinuria without diabetes. For the longitudinal validation, we recruited 563 diabetic patients and collected 1363 urine samples with the clinical data during a follow-up of 6 years. Comparative urinary proteomics identified four proteins Apolipoprotein A-I (APOA1), Beta-2-microglobulin (B2M), E-cadherin (CDH1) and Lithostathine-1-alpha (REG1A), which differentiated with high statistical strength ($p$ < 0.05) between DNpatients and the other groups. Label-freemass spectrometric quantification of the candidates confirmed the discriminatory value of E-cadherin and Lithostathine-1-alpha ($p$ < 0.05). Immunological validation highlighted E-cadherin as the only marker able to differentiate significantly between the different DN stages with an area under the curve (AUC) of 0.85 (95%-CI: [0.72, 0.97]). The analysis of the samples from the longitudinal study confirmed the prognostic value of E-cadherin, the critical increase in urinary E-cadherin level was measured 20 $\pm $ 12.5 months before the onset of microalbuminuria and correlated significantly ($p$ < 0.05) with the glomerular filtration rate measured by estimated glomerular filtration rate (eGFR).de
dc.description.sponsorshipOpen-Access-Publikationsfonds 2020
dc.language.isoengde
dc.rightsopenAccess
dc.rightsNamensnennung 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectDiabetic nephropathy; early diagnosis/prognosis; E-cadherin; early biomarkerde
dc.subject.ddc610
dc.titleUrine E-cadherin: A Marker for Early Detection of Kidney Injury in Diabetic Patientsde
dc.typejournalArticlede
dc.identifier.doi10.3390/jcm9030639
dc.type.versionpublishedVersionde
dc.relation.eISSN2077-0383
dc.bibliographicCitation.volume9de
dc.bibliographicCitation.issue3de
dc.bibliographicCitation.firstPage1de
dc.bibliographicCitation.lastPage21de
dc.type.subtypejournalArticle
dc.bibliographicCitation.articlenumber639de
dc.description.statuspeerReviewedde
dc.bibliographicCitation.journalJournal of Clinical Medicinede


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