Show simple item record

Proteome profiling in murine models of multiple sclerosis: identification of stage specific markers and culprits for tissue damage.

dc.contributor.authorLinker, Ralf A.
dc.contributor.authorBrechlin, Peter
dc.contributor.authorJesse, Sarah
dc.contributor.authorSteinacker, Petra
dc.contributor.authorLee, D. H.
dc.contributor.authorAsif, Abdul R.
dc.contributor.authorJahn, Olaf
dc.contributor.authorTumani, Hayrettin
dc.contributor.authorGold, Ralf
dc.contributor.authorOtto, Markus
dc.date.accessioned2010-12-17T10:46:17Z
dc.date.available2010-12-17T10:46:17Z
dc.date.issued2009-10-28
dc.identifier.citationLinker, Ralf A; Brechlin, Peter; Jesse, Sarah; Steinacker, Petra; Lee, D H; Asif, Abdul R; Jahn, Olaf; Tumani, Hayrettin; Gold, Ralf; Otto, Markus (2009): Proteome profiling in murine models of multiple sclerosis: identification of stage specific markers and culprits for tissue damage. - PloS one, Vol. 4, Nr. 10, p. e7624
dc.relation.ISSN1932-6203
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/5819
dc.description.abstractThe identification of new biomarkers is of high interest for the prediction of the disease course and also for the identification of pathomechanisms in multiple sclerosis (MS). To specify markers of the chronic disease phase, we performed proteome profiling during the later phase of myelin oligodendrocyte glycoprotein induced experimental autoimmune encephalomyelitis (MOG-EAE, day 35 after immunization) as a model disease mimicking many aspects of secondary progressive MS. In comparison to healthy controls, high resolution 2 dimensional gel electrophoresis revealed a number of regulated proteins, among them glial fibrilary acidic protein (GFAP). Phase specific up-regulation of GFAP in chronic EAE was confirmed by western blotting and immunohistochemistry. Protein levels of GFAP were also increased in the cerebrospinal fluid of MS patients with specificity for the secondary progressive disease phase. In a next step, proteome profiling of an EAE model with enhanced degenerative mechanisms revealed regulation of alpha-internexin, syntaxin binding protein 1, annexin V and glutamate decarboxylase in the ciliary neurotrophic factor (CNTF) knockout mouse. The identification of these proteins implicate an increased apoptosis and enhanced axonal disintegration and correlate well the described pattern of tissue injury in CNTF -/- mice which involve oligodendrocyte (OL) apoptosis and axonal injury.In summary, our findings underscore the value of proteome analyses as screening method for stage specific biomarkers and for the identification of new culprits for tissue damage in chronic autoimmune demyelination.
dc.format.extent9
dc.language.isoeng
dc.rightsopenAccess
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/
dc.subjectGene Expression Profiling; Gene Expression Regulation; Mice; Mice, Inbred C57BL; Mice, Transgenic; Multiple Sclerosis/genetics; Multiple Sclerosis/metabolism; Oligodendroglia/pathology; Proteome; Proteomics/methods
dc.subject.ddc610
dc.subject.meshAnimals
dc.subject.meshApoptosis
dc.subject.meshAxons
dc.subject.meshDisease Models, Animal
dc.subject.meshEncephalomyelitis, Autoimmune, Experimental
dc.subject.meshGene Expression Profiling
dc.subject.meshGene Expression Regulation
dc.subject.meshMice
dc.subject.meshMice, Inbred C57BL
dc.subject.meshMice, Transgenic
dc.subject.meshMultiple Sclerosis
dc.subject.meshOligodendroglia
dc.subject.meshProteome
dc.subject.meshProteomics
dc.subject.meshTime Factors
dc.titleProteome profiling in murine models of multiple sclerosis: identification of stage specific markers and culprits for tissue damage.
dc.typejournalArticle
dc.identifier.doi10.1371/journal.pone.0007624
dc.type.versionpublishedVersion
dc.identifier.fs544326
dc.bibliographicCitation.volume4
dc.bibliographicCitation.issue10
dc.type.subtypejournalArticle
dc.identifier.pmid19865482
dc.bibliographicCitation.articlenumbere7624
dc.description.statuspeerReviewed
dc.bibliographicCitation.journalPloS one


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

These documents are avalilable under the license:
openAccess