Show simple item record

Superresolution microscopy in heart — Cardiac nanoscopy

dc.contributor.authorKohl, Tobias
dc.contributor.authorWestphal, Volker
dc.contributor.authorHell, Stefan W.
dc.contributor.authorLehnart, Stephan E.
dc.date.accessioned2013-05-15T06:56:46Z
dc.date.available2013-05-15T06:56:46Z
dc.date.issued2012
dc.identifier.citationKohl, Tobias; Westphal, Volker; Hell, Stefan W.; Lehnart, Stephan E. (2012): Superresolution microscopy in heart — Cardiac nanoscopy
dc.relation.ISSN0022-2828
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?gs-1/8981
dc.description.abstractDetailed understanding of the adaptive nature of cardiac cells in health and disease requires investigation of proteins and membranes in their native physiological environment, ideally by noninvasive optical methods. However, conventional light microscopy does not resolve the spatial characteristics of small fluorescently labeled protein or membrane structures in cells. Due to diffraction limiting resolution to half the wavelength of light, adjacent fluorescent molecules spaced at less than ~250 nm are not separately visualized. This fundamental problem has lead to a rapidly growing area of research, superresolution fluorescence microscopy, also called nanoscopy. We discuss pioneering applications of superresolution microscopy relevant to the heart, emphasizing different nanoscopy strategies towards new insight in cardiac cell biology. Here, we focus on molecular and structural readouts from subcellular nanodomains and organelles related to Ca2+ signaling during excitationcontraction (EC) coupling, including live cell imaging strategies. Based on existing data and superresolution techniques, we suggest that an important future aim will be subcellular in situ structure-function analysis with nanometric resolving power in organotypic cells.
dc.language.isoeng
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/2009-241526
dc.rightsopenAccess
dc.subjectCalcium; excitation-contraction coupling; cell biology; heart failure; superresolution; fluorescence microscopy
dc.titleSuperresolution microscopy in heart — Cardiac nanoscopy
dc.typejournalArticle
dc.identifier.doi10.1016/j.yjmcc.2012.11.016
dc.type.versionsubmittedVersion
dc.identifier.fs600133
dc.relation.pISSN00222828
dc.bibliographicCitation.volume58
dc.bibliographicCitation.firstPage13
dc.bibliographicCitation.lastPage21
dc.type.subtypejournalArticle
dc.identifier.pmid23219451
dc.relation.euprojectEUTrigTreat
dc.description.statuspeerReviewed
dc.bibliographicCitation.journalJournal of Molecular and Cellular Cardiology


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record